Revista de Investigación y Desarrollo Pesquero 2016 Nro 29

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Evaluación de la inmovilidad tónica como técnica para minimizar el estrés de manejo de cautiverio de chuchos (Myliobatis goodei) mediante la medición de lactato en sangre
(Instituto Nacional de Investigación y Desarrollo Pesquero, 2016) Jañez, Julieta; Abraham, Carolina; Palmerio, Andrés; Argemi, Federico
Tonic immobility (TI) is an innate and reversible response to stress observed in a large number of taxa that includes elasmobranches. Although in aquariums it constitutes a safe tool in animal handling procedures, little is known about its physiological effect. Blood lactate concentration is used as an indicator of the stress derived from elasmobranches catch and handling. The aim of this study was to evaluate the response to stress produced in Myliobatis goodie using TI as a handling tool. Thirteen specimens were subject to constant and interrupted TI during which blood samples were taken at 0, 10, 30, 60, 90 and 120 min interval. It was observed that in TIconst lactate concentration (mmol l-1) remains more stable through time (t0 = 1.23; t10 = 2.33; t30 = 3.23; t60 = 3.55; t90 = 4.48; t120 = 5.3). TIint showed that each procedure generates an increase in levels (t0 = 1.17; t10 = 2.19; t30 = 3.96; t60 = 5.04; t90 = 8.10; t120 = 8.42). Results indicate that TIconst prevents significant increments in blood lactate levels; thus, it can be considered a useful tool to be used in procedures that require to secure and retain organisms for long periods of time.